Dr. Soon-Hwan Kwon, Ph.D., Armed Forces Medical Research Institute (AFMRI), gave a lecture entitled “Analysis of adenovirus genotypes and neutralization effects between genotypes” as part of the 2019 IVI Seminar Series: The Present and Future of Vaccine Development, at IVI headquarters in Seoul on April 29.
Title: Analysis of adenovirus genotypes and neutralization effects between genotypes
Human adenoviruses (HAdV) are non-enveloped, double-stranded DNA viruses belonging to the Adenoviridae family. HAdV can cause a wide spectrum of clinical symptoms including upper/lower respiratory tract illness. HAdV has resulted in outbreaks in many countries, including China, France, Israel and Korea. In this study, we developed a type-specific diagnostic method for HAdV using chip-based ultra-fast-PCR (UF-PCR) and established a plaque reduction neutralization test (PRNT) assay for HAdV type 4, 7, and 55. For the type-specific diagnostic method of HAdV, sequences of HAdV type 1, 2, 3, 4, 5, 6, 7, and 55 were analyzed and the unique nucleotide sequences were selected to establish the multiplex UF-PCR conditions. We performed the multiplex UF-PCR with type-specific primers and obtained the results within 20 minutes. Based on the results, chip-based UF-PCR is expected to be available for field diagnosis and initial management of viral outbreaks. Recently, HAdV type 55, a rearrangement of HAdV types 11 and 14, was reported to cause severe community-acquired pneumonia in the Korean military. Here, we established PRNT for the neutralizing effect between adenovirus subtypes (type 4, 7, and 55). In the results, anti-HAdV type 55 serum neutralized against HAdV type 55 but does not protect against HAdV types 4 and 7 in A540 cells. The results showed that the previous HAdV type 4 and 7 vaccine cannot prevent HAdV type 55 infection. Therefore there is a need to develop a new vaccine for HAdV type 55.